Health & Environmental Research Online (HERO)


Print Feedback Export to File
629302 
Journal Article 
ATP depletion precedes the onset and progression of cell injury by paracetamol, 2,4-dinitrophenol, and ethionine in rat liver slices 
Martin, FL; McLean, AE 
1996 
Yes 
In Vitro Toxicology
ISSN: 0888-319X 
9 
2 
191-199 
We have investigated the relationship between ATP levels and the onset and progression of cell injury induced by three chemicals, each exerting toxicity via a different mechanism. Rat liver slices were used in a model in vitro system to investigate the time-course of paracetamol, 2,4-dinitrophenol (DNP), and ethionine injury. Slices from phenobarbitone-induced rats were exposed to 10 mM paracetamol for 120 min and, then, incubated without paracetamol for up to 240 min. Slices from non-induced rats were exposed to DNP (50 muM) or ethionine (10 mM) for up to 240 min. Leakage of lactate dehydrogenase (IDH) and potassium (K+) quantified cell injury. ATP levels were measured using the luciferin-luciferase bioluminescence assay. This study shows that although the time-course of the onset and progression of cell injury induced by each chemical is different, there are still similarities to be found. ATP depletion precedes irreversible cell injury induced by exposure to paraceta