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HERO ID
6304375
Reference Type
Journal Article
Title
The direct inhibitory effect of dutasteride or finasteride on androgen receptor activity is cell line specific
Author(s)
Chhipa, RR; Halim, D; Cheng, J; Zhang, HY; Mohler, JL; Ip, C; Wu, Y
Year
2013
Is Peer Reviewed?
1
Journal
Prostate
ISSN:
0270-4137
EISSN:
1097-0045
Volume
73
Issue
14
Page Numbers
1483-1494
Language
English
PMID
23813737
DOI
10.1002/pros.22696
Web of Science Id
WOS:000323450800001
Abstract
BACKGROUND:
Finasteride and dutasteride were developed originally as 5α-reductase inhibitors to block the conversion of testosterone to dihydrotestosterone (DHT). These drugs may possess off-target effects on the androgen receptor (AR) due to their structural similarity to DHT.
METHODS:
A total of four human prostate cancer cell models were examined: LNCaP (T877A mutant AR), 22Rv1 (H874Y mutant AR), LAPC4 (wild-type AR), and VCaP (wild-type AR). Cells were cultured in 10% charcoal-stripped fetal bovine serum, either with or without DHT added to the medium. AR activity was evaluated using the ARE-luciferase assay or the expression of AR regulated genes.
RESULTS:
Dutasteride was more potent than finasteride in interfering with DHT-stimulated AR signaling. Disruption of AR function was accompanied by decreased cell growth. Cells that rely on DHT for protection against death were particularly vulnerable to dutasteride. Different prostate cancer cell models exhibited different sensitivities to dutasteride and finasteride. LNCaP was most sensitive, LAPC4 and VCaP were intermediate, while 22Rv1 was least sensitive. Regardless of the AR genotype, if AR was transfected into drug-sensitive cells, AR was inhibited by drug treatment; and if AR was transfected into drug-resistant cells, AR was not inhibited.
CONCLUSIONS:
The direct inhibitory effect of dutasteride or finasteride on AR signaling is cell line specific. Mutations in the ligand binding domain of AR do not appear to play a significant role in influencing the AR antagonistic effect of these drugs. Subcellular constituent is an important factor in determining the drug effect on AR function.
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