Tethered-liquid omniphobic surface coating reduces surface thrombogenicity, delays clot formation and decreases clot strength ex vivo | Health & Environmental Research Online (HERO)

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Citation
Tags

HERO ID

6320220

Reference Type

Journal Article

Title

Tethered-liquid omniphobic surface coating reduces surface thrombogenicity, delays clot formation and decreases clot strength ex vivo

Author(s)

Roberts, TR; Leslie, DC; Cap, AP; Cancio, LC; Batchinsky, AI

Year

2020

Is Peer Reviewed?

Yes

Journal

Journal of Biomedical Materials Research. Part B: Applied Biomaterials
ISSN: 1552-4973
EISSN: 1552-4981

Volume

108

Issue

Page Numbers

496-502

Language

English

PMID

31069955

DOI

10.1002/jbm.b.34406

Web of Science Id

WOS:000505634000018

Abstract

Hemocompatible materials for extracorporeal life support (ECLS) technology are investigated to mitigate thrombotic complications associated with this therapy. A promising solution is an omniphobic bilayer coating, tethered liquid perfluorocarbon (TLP), which utilizes an immobilized tether to anchor a mobile, liquid surface lubricant that prevents adhesion of blood components to the substrate. In this study, we investigated the effects of TLP on real-time clot formation using thromboelastography (TEG). TLP was applied to TEG cups, utilizing perfluorodecalin (PFD) or FluorLube63 as the liquid layer, and compared to uncoated cups. Human blood (n = 10) was added to cups; and TEG parameters (R, K, α-angle, MA, LY30, LY60) and adherent thrombus weight were assessed. TLP decreased clot amplification (α-angle), clot strength (MA), and adherent clot weight (p < .0001). These effects were greater with FluorLube63 versus PFD (α-angle p < .0001; MA p = .0019; clot weight p < .0001). Reaction time (R) was longer in TLP-coated cups versus control cups with liquid lubricant added (p = .0377). Percent fibrinolysis (LY30 and LY60) was greater in the TLP versus controls at LY30 (p < .0001), and in FluoroLube63 versus controls at LY60 (p = .0021). TLP significantly altered clot formation, exerting antithrombogenic effects. This reduction in surface thrombogenicity supports TLP as a candidate for improved biocompatibility of ECLS materials, pending further validation with exposure to shear stress.

Keywords

Medical Sciences; anticoagulation; antithrombogenic surface; biocompatibility; biomaterials; Perfluorodecalin; Shear stress; Perfluorocarbons; Liquid surfaces; Fibrinolysis; Substrates; Reaction time; Complications; Coatings; Thrombosis