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6323953 
Journal Article 
Toxicological studies with sodium cyclamate and saccharin 
Taylor, JD; Richards, RK; Wiegand, RG; Weinberg, MS 
1968 
1 
Food and Cosmetics Toxicology
ISSN: 0015-6264
EISSN: 1878-6049 
6 
3 
313-327 
The effects of acute, subacute and chronic administration of sodium cyclamate, sodium saccharin or combinations of cyclamate and saccharin have been studied in mice, rats and dogs. The acute toxicity of the materials was shown to vary with the strain of animal and the fat content of the diet. The combination was less toxic to Wistar rats than either of the components. There were no significant effects upon the susceptibility of young mice to the acute intraperitoneal effects of the sweeteners when cyclamate was administered to the gestating dam throughout gestation. In subacute studies there were no effects with administration to rats of up to 2% cyclamates in the diet, or of a mixture of cyclamate and saccharin (10:1, by weight) either at a level of 2% in the diet or intragastrically in doses of 1 g/kg/day. Intragastric administration of cyclamate to dogs at a level of 4 g/kg/day was also without effect. Reduced food intake was noted at high levels in some rat studies. In 11-month studies, there were no effects from administration of as much as 3% sodium cyclamate in the diet to rats or as much as 0.5 g/kg body weight/day to dogs (other than occasional stool-softening). The mating, parturition and weaning data for three generations raised from a parent generation of treated rats and maintained on test diets after weaning indicated that sodium cyclamate has no effect on reproductive performance. Previous literature reports show the absence of pharmacological effect. The stool-softening effect of high levels of cyclamate intake is attributable to the osmotic action of this salt in the bowel, reducing the customary dehydration of faeces in a manner similar to that of sodium sulphate. Some recent reports alleging potentially dangerous effects of the cyclamates may be viewed as representing non-germaine findings in animals, since the dosage levels are equivalent to human intakes far in excess of 100 g/day. No significant effects have been seen in these or any other studies at levels of intake that would be meaningful in relation to man.