US EPA

6332245 

Journal Article 

Proteomic analysis of the hepatotoxicity of Microcystis aeruginosa in adult zebrafish (Danio rerio) and its potential mechanisms 

Du, B; Liu, G; Ke, M; Zhang, Z; Zheng, M; Lu, T; Sun, L; Qian, H 

2019 

Yes 

Environmental Pollution
ISSN: 0269-7491
EISSN: 1873-6424 

Elsevier Ltd 

254 

Pt A 

113019 

English 

31419664 

10.1016/j.envpol.2019.113019 

WOS:000488887500043 

Microcystis aeruginosa is one of the main species of cyanobacteria that causes water blooms. M. aeruginosa can release into the water several types of microcystins (MCs), which are harmful to aquatic organisms and even humans. However, few studies have investigated the hepatotoxicity of M. aeruginosa itself in zebrafish in environments that simulate natural aquatic systems. The objective of this study was to evaluate the hepatotoxicity of M. aeruginosa in adult zebrafish (Danio rerio) after short-term (96 h) exposure and to elucidate the potential underlying mechanisms. Distinct histological changes in the liver, such as enlargement of the peripheral nuclei and sinusoids and the appearance of fibroblasts, were observed in zebrafish grown in M. aeruginosa culture. In addition, antioxidant enzyme activity was activated and protein phosphatase (PP) activity was significantly decreased with increasing microalgal density. A proteomic analysis revealed alterations in a number of protein pathways, including ribosome translation, immune response, energy metabolism and oxidative phosphorylation pathways. Western blot and real-time PCR analyses confirmed the results of the proteomic analysis. All results indicated that M. aeruginosa could disrupt hepatic functions in adult zebrafish, thus highlighting the necessity of ecotoxicity assessments for M. aeruginosa at environmentally relevant densities. 

Gene expression; Hepatotoxicity; Microcystis aeruginosa; Proteomic; Western blot; Zebrafish; Bacteria; Cell culture; Enzyme activity; Gene expression; Metabolism; Polymerase chain reaction; Hepatotoxicity; Microcystis aeruginosa; Proteomic; Western blots; Zebrafish; Aquatic organisms; antioxidant; microcystin; phosphoprotein phosphatase; antioxidant; microcystin; proteome; zebrafish protein; algal bloom; cyanobacterium; cyprinid; enzyme activity; gene expression; polymerase chain reaction; proteomics; toxicity; adult; animal cell; animal experiment; animal model; animal tissue; Article; bacterium culture; cell nucleus; controlled study; ecotoxicity; energy metabolism; enzyme activation; enzyme activity; fibroblast; histopathology; immune response; liver cell; liver sinusoid; liver toxicity; Microcystis aeruginosa; nonhuman; oxidative phosphorylation; protein metabolism; proteomics; real time polymerase chain reaction; ribosome; RNA translation; Western blotting; zebra fish; animal; cyanobacterium; human; metabolism; Microcystis; physiology; proteomics; toxic hepatitis; toxicity; water pollutant; Cyanobacteria; Danio rerio; Microcystis aeruginosa; Animals; Antioxidants; Chemical and Drug Induced Liver Injury; Cyanobacteria; Humans; Microcystins; Microcystis; Proteome; Proteomics; Water Pollutants; Zebrafish; Zebrafish Proteins