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6351003 
Data & Software 
Computer Program 
S-Ibuprofen Effectively Inhibits Thromboxane B2 Levels and Platelet Function in an Experimental Model of Lipopolysaccharide-Stimulated and Non-Stimulated Whole Blood 
Kaehler, S; Marsik, C; Heinisch, B; Thallinger, C; Sauermann, R; Kazemi-Shirazi, L; Wagner, O; Joukhadar, C 
2008 
1 
Pharmacology
ISSN: 0031-7012
EISSN: 1423-0313 
81 
2 
181-186 
English 
18043009 
WOS:000251436700014 
OBJECTIVEThe present study aimed at testing the effect of S- and R-ibuprofen on thromboxane B(2) (TXB(2)), collagen-epinephrine closure time (CEPI-CT) and collagen-adenosine 5'-diphosphate closure time (CADP-CT) in lipopolysaccharide (LPS)-stimulated and non-stimulated human whole blood.MATERIALS AND METHODSWhole blood was incubated with S- or R-ibuprofen with and without prior stimulation with LPS. To verify ibuprofen's potential effects on TXB(2), varying ratios of concentrations of S- and R-ibuprofen ranging from 0 to 100% were used. TXB(2) levels were measured by ELISA. The effects of S- and R-ibuprofen enantiomers on platelet aggregability were tested utilizing a PFA-100 apparatus.RESULTSIn non-stimulated and LPS-stimulated whole blood, S-ibuprofen markedly decreased TXB(2) levels at concentrations ranging from 10 to 200 microg/ml. R-ibuprofen showed its inhibiting effect at concentrations >100 microg/ml. In inflammatory and non-inflammatory conditions, CEPI-CT was prolonged at concentrations of 12.5 and 75 microg/ml for S-ibuprofen and at a concentration of 150 microg/ml of combined R- and S-ibuprofen. S-ibuprofen was significantly more effective than R-ibuprofen (p < 0.05). The combined use of S- and R-ibuprofen did not additively or synergistically prolong CEPI-CTs. CADP-CTs remained unaffected by both enantiomers.CONCLUSIONSS-ibuprofen was more effective than the R-ibuprofen enantiomer in inhibiting TXB(2) plasma levels and aggregability of thrombocytes in non-inflammatory and inflammatory conditions. 
Adult; Dose-Response Relationship; Ibuprofen/chemistry; Ibuprofen/pharmacology; Lipopolysaccharides/toxicity; Platelet Aggregation/drug effects; Platelet Aggregation/physiology; Platelet Function Tests/methods; Stereoisomerism; Thromboxane B2/antagonists & inhibitors; Thromboxane B2/blood; Thromboxane B2/secretion; Whole Blood Coagulation Time/methods