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6401887 
Journal Article 
Increasing dietary EPA and DHA influence estimated fatty acid desaturase activity in systemic organs which is reflected in the red blood cell in mice 
Davidson, EA; Pickens, CA; Fenton, JI 
2018 
Yes 
International Journal of Food Sciences and Nutrition
ISSN: 0963-7486
EISSN: 1465-3478 
69 
2 
183-191 
English 
28697636 
WOS:000423428500006 
Delta-5 (D5D) and delta-6 (D6D) desaturase are key enzymes in fatty acid (FA) metabolism. Dietary eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) may alter tissue FA composition via D5D and D6D. The purpose was to determine the relationship between dietary EPA + DHA, estimated desaturase activities of various tissues and the reflection of desaturase activity in the red blood cell (RBC). Mice were fed diets with increasing percent of energy from EPA + DHA. Phospholipid FA composition of heart, muscle, spleen, lung, adipose tissues and RBC were analysed. D5D and D6D enzyme activity estimates (EAE) were calculated as the ratio of 20:4/20:3 and 20:3/18:2, respectively. D5D EAE decreased in all tissues, except muscle, with increasing dietary EPA + DHA. RBC D5D EAE positively correlated with D5D EAE in all tissues. RBC D6D EAE positively correlated with muscle and inversely correlated with adipose D6D EAE. Our findings suggest differential influence of dietary EPA + DHA upon tissue desaturase activities. 
; Blood; Diet; Muscles; Eicosapentaenoic acid; Metabolism; Spleen; Fatty acids; Organs; Cardiac muscle; Red blood cells; Desaturase; Correlation; Phospholipids; Adipose tissue; Enzymatic activity; Heart; Animal tissues; Enzyme activity; Docosahexaenoic acid; Lungs/