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6410021 
Journal Article 
Engineering resistance against tomato yellow leaf curl virus (TYLCV) using antisense RNA 
Bendahmane, M; Gronenborn, B 
1997 
1 
Plant Molecular Biology
ISSN: 0167-4412
EISSN: 1573-5028 
33 
2 
351-357 
English 
9037152 
One of the most severe diseases of cultivated tomato worldwide is caused by tomato yellow leaf curl virus (TYLCV), a geminivirus transmitted by the whitefly Bemisia tabaci. Here we describe the application of antisense RNAs to interfere with the disease caused by TYLCV. The target of the antisense RNA is the rare messenger RNA of the Rep protein, encoded by the C1 gene. Transgenic Nicotiana benthamiana plants expressing C1 antisense RNA were obtained and shown to resist infection by TYLCV. Some of the resistant lines are symptomless, and the replication of challenge TYLCV almost completely suppressed. The transgenes mediating resistance were shown to be effective through at least two generations of progeny. 
; Animals; Base Sequence; DNA Helicases/biosynthesis; DNA Helicases/genetics; DNA-Binding Proteins; Diptera/virology; Geminiviridae/genetics; Geminiviridae/pathogenicity; Genes; Viral; Lycopersicon esculentum/virology; Molecular Sequence Data; Plant Diseases/virology; Plants; Genetically Modified; Plants; Toxic; RNA; Antisense/biosynthesis; RNA; Messenger/antagonists & inhibitors; RNA; Messenger/biosynthesis; Restriction Mapping; Tobacco; Trans-Activators/biosynthesis; Trans-Activators/genetics; Viral Proteins/genetics; Index Medicus/