US EPA

6419867 

Journal Article 

Dehydrozingerone, a structural analogue of curcumin, induces cell-cycle arrest at the G2/M phase and accumulates intracellular ROS in HT-29 human colon cancer cells 

Yogosawa, S; Yamada, Y; Yasuda, S; Sun, Q; Takizawa, K; Sakai, T 

2012 

Yes 

Journal of Natural Products
ISSN: 0163-3864
EISSN: 1520-6025 

75 

12 

2088-2093 

English 

23245566 

10.1021/np300465f 

Dehydrozingerone (1) is a pungent constituent present in the rhizomes of ginger (Zingiber officinale) and belongs structurally to the vanillyl ketone class. It is a representative of half the chemical structure of curcumin (2), which is an antioxidative yellow pigment obtained from the rhizomes of turmeric (Curcuma longa). Numerous studies have suggested that 2 is a promising phytochemical for the inhibition of malignant tumors, including colon cancer. On the other hand, there have been few studies on the potential antineoplastic properties of 1, and its mode of action based on a molecular mechanism is little known. Therefore, the antiproliferative effects of 1 were evaluated against HT-29 human colon cancer cells, and it was found that 1 dose-dependently inhibited growth at the G2/M phase with up-regulation of p21. Dehydrozingerone additionally led to the accumulation of intracellular ROS, although most radical scavengers could not clearly repress the cell-cycle arrest at the G2/M phase. Furthermore, two synthetic isomers of 1 (iso-dehydrozingerone, 3, and ortho-dehydrozingerone, 4) were also examined. On comparing of their activities, accumulation of intracellular ROS was found to be interrelated with growth-inhibitory effects. These results suggest that analogues of 1 may be potential chemotherapeutic agents for colon cancer. 

; Curcuma longa; Zingiber officinale; antineoplastic activity; cell cycle checkpoints; colorectal neoplasms; curcumin; drug therapy; free radical scavengers; ginger; growth retardation; humans; isomers; mechanism of action; neoplasm cells; rhizomes; turmeric/