Health & Environmental Research Online (HERO)


Print Feedback Export to File
6554726 
Journal Article 
Estrogenicity of 4-nonylphenol and diethylstilbestrol on in vitro oocyte maturation of the dusky tripletooth goby, Tridentiger obscurus 
Hwang, IJ; Kim, HW; Kim, JK; Lee, YD; Baek, HJ 
2010 
Yes 
Animal Cells and Systems
ISSN: 1976-8354 
14 
3 
161-167 
English 
WOS:000281763200003 
This study investigated the estrogenicity of 4-nonylphenol (NP) and diethylstilbestrol (DES) in vitro during oocyte maturation in the marine fish, Tridentiger obscurus, using steroid hormone assays and GVBD assay. Vitellogenic (0.53mm diameter) and fully vitellogenic (0.75mm diameter) oocytes were in vitro exposed to NP (0.045-453.82 nM) and DES (0.037-372.62 nM). In vitellogenic oocytes, 45.38 and 453.82nM NP and 3.73-372.62nM DES increased the estradiol-17 beta (E2)/testosterone (T) ratio. In fully vitellogenic oocytes, 0.45, 45.38 and 453.82nM NP and 3.73nM DES increased E2/T. In the GVBD assay, 0.45 and 4.54nM NP and 0.037, 3.73 and 37.26nM DES inhibited GVBD. These results suggest that NP and DES have estrogen-agonistic effects in oocyte maturation in T. obscurus. In addition, NP and DES have different sensitivity according to the oocyte developmental stage, and the estrogen-agonistic effects of DES were greater than were those of NP. 
diethylstilbestrol; dusky tripletooth goby; estrogenicity; 4-nonylphenol; oocyte maturation