Management of public health in close correlation with food safety is a challenge nowadays, when human society is continually developing. Synthetic food additives are a compromise to natural food, having high toxicity on all viable organisms. Through their technology of synthesis, manufacturing and then discharging into the environment emerged imbalances in human health for short term, but at the same time, have resulted some serious environmental problems in the medium and long term. This requires careful management of these synthesis products. The aim of the present study was to determine toxic influence of two of the most commonly synthetic food dyes used in the food industry, tartrazine (E102) and carmoisine (E122), in some alive tissues from plants and filamentous fungi. This procedure can be used as a primary test method before testing on animals or humans organism. Thus, knowing how it reacts with the various biotic and abiotic structures in the environment, it creates an operational framework for the degradation of these compounds. At laboratory conditions, the test organisms species were Lepidium sativum, fast-growing plant and bio-indicator and Penicillium notatum fungus endowed with a complex enzyme capacity. Substance doses for toxicity testing were diluted by successive halving starting at 100% initial concentration and decreasing to 50, 25, 12.5, 6.25, and 0% (distilled water), for each type of aqueous dye solutions, respectively. Quantification of toxic response was taking into account specific inhibition rate (I%) following the interaction between test substances and organisms. To determine specific inhibition (I%) the toxic response was transposed through some morpho-physiological parameters such as: germination rate (%), root length (mm), stem length (mm), vigor index for plants, and the inhibition zone on the fungal colonies (mm) in Petri dishes. The concentration-dependent inhibition on the organisms viability was noted at 24, 48 and 72 h of the exposure. In both cases, the results suggest that an inhibition of the optimum physiological parameters of living organisms was induced as the concentrations of the tested substances increase.<