US EPA

658628 

Journal Article 

The ECVAM International Validation Study on in vitro tests for skin corrosivity. 2. Results and evaluation by the management team 

Fentem, JH; Archer, GE; Balls, M; Botham, PA; Curren, RD; Earl, LK; Esdaile, DJ; Holzhuetter, HG; Liebsch, M 

1998 

1 

Toxicology In Vitro
ISSN: 0887-2333
EISSN: 1879-3177 

BIOSIS/98/30489 

Toxicology In Vitro 

12 

4 

483-524 

English 

As a follow-up to a prevalidation study on in vitro tests for replacing the in vivo rabbit test for skin corrosivity, an international validation study was conducted during 1996 and 1997 under the auspices of ECVAM. The main objectives of the study were to: (a) identify tests capable of discriminating corrosives from non-corrosives for selected types of chemicals and/or all chemicals; and (b) determine whether these tests could identify correctly known R35 (UN packing group I) and R34 (UN packing groups II & III) chemicals. The tests evaluated were the rat skin transcutaneous electrical resistance (TER) assay, CORROSITEX, the SKIN2TM ZK1350 corrosivity test and EPISKINTM. Each test was conducted in three independent laboratories. 60 coded chemicals were tested. All of the tests evaluated showed acceptable intralaboratory and interlaboratory reproducibilities, and the TER, Skin2 and EPISKIN tests proved applicable to testing a diverse group of chemicals of different phy 

Biochemistry methods; Biochemistry; Biophysics methods; Biophysics; Macromolecular systems; Molecular biology; Diagnosis; Skin; Poisoning; Animals; laboratory; Muridae; 61790-33-8; 10026-13-8; 7789-60-8; 7705-08-0; 7664-93-9; 7664-38-2; 7647-01-0; 7631-90-5; 5638-12-0; 5329-14-6; 3446-89-7; 3398-33-2; 2550-26-7; 2370-63-0; 1647-16-1; 1310-58-3; 996-35-0; 590-01-2; 584-13-4; 499-75-2; 497-19-8; 334-48-5; 151-21-3; 144-55-8; 143-07-7; 142-62-1; 140-31-8; 127-18-4; 124-07-2; 118-82-1; 116-53-0; 112-39-0; 112-38-9; 111-82-0; 111-68-2; 106-95-6; 104-55-2; 103-63-9; 97-53-0; 95-68-1; 90-05-1; 88-18-6; 78-90-0; 78-85-3; 78-76-2; 67-63-0; 60-12-8