A screening system for the directed evolution of epoxygenases: Importance of position 184 in P450 BM3 for stereoselective styrene epoxidation | Health & Environmental Research Online (HERO)

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Citation
Tags

HERO ID

6594430

Reference Type

Journal Article

Title

A screening system for the directed evolution of epoxygenases: Importance of position 184 in P450 BM3 for stereoselective styrene epoxidation

Author(s)

Tee, K; Schwaneberg, U; ,

Year

2006

Is Peer Reviewed?

Yes

Journal

Angewandte Chemie (International Edition)
ISSN: 1433-7851
EISSN: 1521-3773

Publisher

WILEY-V C H VERLAG GMBH

Location

WEINHEIM

Volume

Issue

Page Numbers

5380-5383

Language

English

PMID

16847856

DOI

10.1002/anie.200600255

Web of Science Id

WOS:000239913400031

URL

https://www.scopus.com/inward/record.uri?eid=2-s2.0-33747885449&doi=10.1002%2fanie.200600255&partnerID=40&md5=9325f8449063dcd9c060bf1f91dc7db1
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Abstract

Positively charged: A screening system for the directed evolution of epoxygenases has been developed. Validation reveals that introducing a positively charged amino acid at position 184 (see picture) results in inversion of enantioselectivity in the epoxidation of styrene. The synthetic potential of epoxygenases can now be tailored by methods of directed evolution. (Figure Presented) © 2006 Wiley-VCH Verlag GmbH &. Co. KGaA.