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6603101 
Journal Article 
ANALYSIS OF LIPID CLASSES BY SOLID-PHASE EXTRACTION AND HIGH-PERFORMANCE SIZE-EXCLUSION CHROMATOGRAPHY 
Hopia, AI; Piironen, VI; Koivistoinen, PE; Hyvonen, LET; , 
1992 
Yes 
Journal of the American Oil Chemists' Society
ISSN: 0003-021X
EISSN: 1558-9331 
AMER OIL CHEMISTS SOC 
CHAMPAIGN 
69 
8 
772-776 
English 
WOS:A1992JJ46000009 
An improved method to analyze lipid classes of edible oils and fats by solid-phase extraction (SPE) and high-performance size-exclusion chromatography (HPSEC) is presented. A mixture of lipid standards was fractionated by the solid-phase extraction procedure (NH2 phase) into polar and nonpolar fractions; these were then submitted to analysis by HPSEC. The size-exclusion chromatographic columns were three styrene/divinylbenzene columns with pore sizes of 100 angstrom and 50 angstrom. Light-scattering was used for the detection system, and the parameters of the detector were optimized to minimize the difference between the responses of the compounds studied. With this procedure it was possible to separate the following lipid classes: triacylglycerols, diacylglycerols, monoacylglycerols and free fatty acids, sterols, sterol esters, tocopherols and carotenoids. Quantitative analysis was studied for a light-scattering detector with several lipid standards of different molecular weights and unsaturation levels.