Health & Environmental Research Online (HERO)


Print Feedback Export to File
6604305 
Journal Article 
Isolation and Characterization of Bacillus megaterium IBBPo17 a Solvent-Tolerant Bacterium 
Stancu, MM; , 
2019 
1 
Waste and Biomass Valorization
ISSN: 1877-2641
EISSN: 1877-265X 
SPRINGER 
DORDRECHT 
10 
12 
3557-3566 
English 
WOS:000496664800002 
A new solvent-tolerant Gram-positive bacterium was isolated from an oily sludge sample. The isolated bacterium was identified based on phenotypic characteristics and 16S rRNA gene sequence as Bacillus megaterium IBBPo17 (GenBank KX499518). Two catabolic genes, such as alkane hydroxylase (alkB1) and naphthalene dioxygenase (ndoM) were detected in the genome of this solvent-tolerant Gram-positive bacterium. B. megaterium IBBPo17 could survive and grow in the presence of toxic organic solvents, such as n-alkanes (n-decane, n-hexane) and cyclic solvents (cyclohexane, ethylbenzene, styrene, toluene). Short-chain n-alkanes (n-hexane, n-decane) with a high logarithm of the partition coefficient of the solvent in octanol-water mixture (log P-OW) were less toxic for B. megaterium IBBPo17, while cyclic solvents (cyclohexane, toluene, styrene, ethylbenzene) with a lower log P-OW were more toxic for this bacterium. B. megaterium IBBPo17 produced both extracellular enzymes (i.e., protease, amylase, cellulase) and extracellular surfactants, which are well-known for their broad potential applications. Changes in the cell ultrastructure, protein and lipid profiles were depicted as an adaptive feature when B. megaterium IBBPo17 cells were exposed to these organic solvents. 
Bacillus megaterium; Mechanisms; Solvent tolerance