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6641621 
Journal Article 
Separation of oligonucleotides on novel monolithic columns with ion-exchange functional surfaces 
Sykora, D; Svec, F; Frechet, JMJ; , 
1999 
ELSEVIER SCIENCE BV 
AMSTERDAM 
297-304 
10480254 
WOS:000082016200031 
Porous monolithic columns have been prepared by the direct free radical copolymerization of glycidyl methacrylate and ethylene dimethacrylate within the confines of a 50x8 mm I.D. chromatographic column in the presence of porogens. The epoxide groups of these monoliths were modified to different extents by reaction with diethylamine to afford 1-N,N-diethylamino-2-hydroxypropyl functionalities useful for ion-exchange chromatography. Following characterization of the monoliths, the columns were tested in the chromatographic separation of a homologous series of oligodeoxyadenylic [pd(A)(12-18)] and oligothymidylic acids [d(pT)(12-24)] at different flow-rates. Very good separations of the oligonucleotides were achieved even at the high flow-rate of 4 ml/min. (C) 1999 Elsevier Science B.V. All rights reserved. 
18th International Symposium on Separation and Analysis of Proteins, Peptides and Polynucleotides (ISPPP 98) 
VIENNA, AUSTRIA