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HERO ID
6643619
Reference Type
Journal Article
Title
HPLC USING DIOL-BONDED SILICA, AN ALTERNATIVE TO SILICA-GEL IN THE PREFRACTIONATION OF AROMA EXTRACTS
Author(s)
Barron, D; ,
Year
1991
Is Peer Reviewed?
1
Journal
Zeitschrift für Lebensmittel-Untersuchung und -Forschung
ISSN:
0044-3026
Publisher
SPRINGER VERLAG
Location
NEW YORK
Page Numbers
454-459
Web of Science Id
WOS:A1991GN11400008
Abstract
A strawberry jam dichloromethane aroma extract was pre-fractionated by normal phase HPLC on diol. This was preceded by neutralization with aqueous sodium bicarbonate, and removal of fatty acids by gel filtration on Sephadex LH-20. Elution of the diol column with a gradient of diethyl ether in pentane resulted in the separation of two main fractions. One contained hydrocarbons and carbonyl compounds, whereas lactones and polar compounds such as hydroxylated carbonyl compounds or alcohols were present in the second fraction. HPLC on diol is highly recommended as the first prefractionation step since, in contrast to silica gel, it allows easy recovery of all aroma constituents.
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