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6643619 
Journal Article 
HPLC USING DIOL-BONDED SILICA, AN ALTERNATIVE TO SILICA-GEL IN THE PREFRACTIONATION OF AROMA EXTRACTS 
Barron, D; , 
1991 
1 
Zeitschrift für Lebensmittel-Untersuchung und -Forschung
ISSN: 0044-3026 
SPRINGER VERLAG 
NEW YORK 
454-459 
WOS:A1991GN11400008 
A strawberry jam dichloromethane aroma extract was pre-fractionated by normal phase HPLC on diol. This was preceded by neutralization with aqueous sodium bicarbonate, and removal of fatty acids by gel filtration on Sephadex LH-20. Elution of the diol column with a gradient of diethyl ether in pentane resulted in the separation of two main fractions. One contained hydrocarbons and carbonyl compounds, whereas lactones and polar compounds such as hydroxylated carbonyl compounds or alcohols were present in the second fraction. HPLC on diol is highly recommended as the first prefractionation step since, in contrast to silica gel, it allows easy recovery of all aroma constituents.