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6659582 
Journal Article 
Application of poly(styrene-divinylbenzene) resin to the reversed phase high performance liquid chromatography for the determination of thymosin alpha 
Shao Cheng-Wei; Wei Rong-Qing; Zhang Ting-Ting; Liu Xiao-Ning; , 
2007 
Yes 
Chinese Journal of Analytical Chemistry / Fen Xi Hua Xue
ISSN: 0253-3820 
ELSEVIER SCIENCE INC 
NEW YORK 
1491-1494 
WOS:000252547500021 
A reversed phase high performance liquid chromatographic( RP-HPLC) method with polystyrene-divinylbenzene (PS-DVB) resin(MKF-RP-MH) was developed for the determination of thymosin alpha(1). In the method, thymosin alpha(1) was chromatographically analyzed with a mobile phase A:0.01 mol/L phosphate buffer solution( pH = 7.0 or 8.0) and B: acetonitrile, linear gradient elution condition: 0-20 min, 0%-10% acetonitrile, 20-40 min, 10%-30% acetonitrile; column temperature was set at 30 degrees C; flow rate was 0.8 mL/min; The detection wavelength was 214 nm. The standard sample and two kinds of thymosin a, samples were analysised under the optimum chromatographic condition. The calibration curve was in good linearity within the range of 0.05-4.0 g/L with the regression equation of C = 0.0099A-0.0798 (r = 0.9994). The maximum amount of thymosin alpha(1) on MKF-RP-MH column was 120 mu g (6 g/L).