US EPA

666380 

Journal Article 

On the carcinogenic activity of dicyclohexylamine and dicyclohexylaminenitrite 

Pliss, GB 

1958 

1 

Voprosy Onkologii
ISSN: 0507-3758 

4 

659-667 

The carcinogenic effects of cyclohexylamine (108918) (CHA), dicyclohexylamine (101837) (DCHA), and dicyclohexylamine-nitrite (3129917) (DCHAN) were studied in rats and mice. D-mice were injected with CHA, DCHA, and DCHAN subcutaneously (sc) to determine their lethal doses (LD50s). Rats received orally 0.5 milliliters (ml) of 5.0 percent CHA and DCHA daily for 12 months. D-mice were given single 0.05ml sc injections of 2.6 percent DCHA. Some animals were also given weekly single sc injections of 0.1ml of 1 percent DCHAN for 12 to 13 months. Rats were given weekly 0.5ml of 2 percent DCHAN sc for 11 to 13 months. The animals were observed for clinical symptoms and the organs of dead and killed animals were examined macroscopically and microscopically. The LD50 values were for CHA, 1.15; DCHA, 0.135; and DCHAN, 0.155 milligrams per gram. No tumors were found in the rats that received CHA. Slight signs of protein dystrophy were seen in liver and kidney. DCHA induced an hepatic tumor in one rat after 21 months and a sarcoma in the omentum in another animal after 22.5 months. Four mice that received DCHA developed sarcomas after 12 months. The tumors were located in the subcutaneous tissue and infiltrated the epidermis. Five mice receiving DCHAN developed tumors after 13 to 20.5 months. The tumors were hepatomas, two pulmonary tumors, a hepatogenic adenoma, and a cavernous hepatohemangioma. Seven rats injected with DCHAN developed tumors, six fibrosarcomas and a pulmonary carcinoma with metastases to the skin. The author concludes that CHA is not carcinogenic, whereas DCHA and DCHAN both have carcinogenic properties. The carcinogenic effects of DCHA and DCHAN are relatively weak, since the tumors developed after 12 months. (Russian)