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667905 
Journal Article 
Effect of saponin on the transmucosal passage of beta-lactoglobulin across the proximal small intestine of normal and beta-lactoglobulin-sensitised rats 
Gee, JM; Wal, JM; Miller, K; Atkinson, H; Grigoriadou, F; Wijnands, MV; Penninks, AH; Wortley, G; Johnson, IT 
1997 
1 
Toxicology
ISSN: 0300-483X
EISSN: 1879-3185 
117 
2-3 
219-228 
BIOSIS COPYRIGHT: BIOL ABS. The ability of saponins and glycoalkaloids to permeabilise the mammalian intestinal barrier has been previously demonstrated in vitro, leading to the hypothesis that membranolytic saponins may facilitate transfer to the tissues of otherwise excluded macromolecules. An enhanced uptake of, for instance, potentially allergenic species from the lumen is one of the factors that may affect the induction of food allergy, and its presentation in already sensitized individuals. In the experiments described here, an increase in the transmucosal uptake of the milk allergen beta-lactoglobulin (betaLG) was assessed in non-sensitized and sensitized Brown Norway rats in the presence of Gypsophila saponin. Isolated jejunal loops were exposed in vivo to either betaLG followed by saponin, saponin followed by betaLG or the two compounds simultaneously. Portal vein blood samples were collected and assayed for betaLG and rat mucosal mast cell protease (RCMP II) activity. Mucosal tissue was 
Digestive system physiology; Digestive system metabolism; Poisoning; Animals; laboratory; Immunity; cellular; Hypersensitivity; Muridae