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668307 
Journal Article 
Detection of viral deoxyribonucleic acid in amniotic fluid: association with fetal malformation and pregnancy abnormalities 
Reddy, U; Baschat, A; Towbin, J; Harman, C; Weiner, C 
2001 
Yes 
American Journal of Obstetrics & Gynecology
ISSN: 0002-9378
EISSN: 1097-6868 
185 
6 Pt 2 
S95 
OBJECTIVE: Determine the relationship between a positive viral polymerase chain reaction in amniotic fluid (AFPCR) and sonographic abnormalities. STUDY DESIGN: PCR testing for CMV, parvovirus B19, adenovirus, enterovirus, herpes simplex virus, Epstein-Barr virus, and respiratory syncytial virus was performed on 423 AF samples obtained for clinical indications. 284 samples were from an East Coast center (ECC) and 139 AF samples from a Midwest center (MC). RESULTS: Gestational age at amniocentesis was similar at the centers (ECC, 19.1 w vs. MC, 20.1 w). Of 423 karyotypically normal fetuses, 57 had a positive AFPCR (13.5%). The most common viruses were adenovirus (77%), enterovirus (12%), CMV and parvovirus (5% each). 33 out of 136 (24%) sonographically abnormal fetuses had a positive viral PCR vs. 24 out of 287 normal fetuses (8.4%), P = .001. Results are summarized in the following two Tables. CONCLUSION: There is no difference in the underlying positive PCR rate for normal fetuses in both locations. A positive PCR is associated with an increased rate of malformations, IUGR, hydrops and other fetal abnormalities. Adenovirus is the most common agent (Table: see text). 
Human; DNA; Viral ANALYSIS; Amniotic Fluid VIROLOGY; Polymerase Chain Reaction; Abnormalities ETIOLOGY; Fetal Growth Retardation ETIOLOGY; Hydrops Fetalis ETIOLOGY; No cas rn