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6694457 
Journal Article 
Hepatocytes primary culture from the neotropical fish, trahira Hoplias malabaricus (Bloch) 
Neto, FF; Zanata, SM; Randi, MAF; Pelletier, E; Ribeiro, CAO 
2006 
Yes 
Journal of Fish Biology
ISSN: 0022-1112
EISSN: 1095-8649 
WILEY 
HOBOKEN 
69 
5 
1524-1532 
WOS:000241730200019 
Hepatocytes were isolated and cultured from Hoplias malabaricus using a non-enzymatic protocol. From 6.9 to 9.4 x 10(7) hepatocytes g(-1) of liver were isolated and cell viability was 65-85%. Fish hepatocytes attached well to untreated polystyrene flasks (Corning) as well as on treated surfaces with extracellular matrix proteins, i.e. fibronectin, matrigel or type I collagen coatings. Cells rapidly started migrating and reorganizing in cord-like groups after seeding, with spaces similar to bile canaliculi in vivo. Prominent nucleolus and euchromatin-rich rounded nucleus, abundant mitochondria, well-developed rough endoplasmic reticulum, polysomes and Golgi apparatus were seen under ultrastructural analysis. Cells remained functional and metabolically active after 6 days in culture. The protocol established in the current work provides the basis for further studies of native fishes for accurate in vitro toxicological studies. (c) 2006 The Authors Journal compilation (c) 2006 The Fisheries Society of the British Isles. 
hepatocytes; Hoplias malabaricus; primary cell culture; tropical fishes