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6717929 
Journal Article 
Infrared Spectrum, UV-visible Absorption Spectral Characteristics and Proteomic Analysis of Selenium Enriched Spirulina Platensis 
Shan Xue-Feng; Zhang Shui-Mei; Cai Zhi-Hui; Ling Qin-Jie; Huang Zhi 
2016 
Yes 
Chinese Journal of Analytical Chemistry / Fen Xi Hua Xue
ISSN: 0253-3820 
Elsevier 
NEW YORK 
44 
12 
1814-1819 
Chinese 
WOS:000393290300003 
Infrared spectrum, UV-visible absorption spectrum and differential expression of proteome between Spirulina platensis (SP) and selenium enriched SP (Se. SP) were investigated. The selenium containing proteins were separated from the total proteins in Se. SP by a proteomic approach of two. dimensional electrophoresis (2DE), identified by matrix assisted laser desorption ionization time. of. flight mass spectrometry (MALDI-TOF-MS), and determined by inductively coupled plasma mass spectrometry (ICP-MS). Results showed that Se enrichment cultivation could significantly up. regulate the expression of pigment proteins such as alpha/beta subunits of phycocyanin (PC) and allophycocyanin (APC) which involved in light harvest. The large subunits of Rbisco and superoxide dismutase were also increased obviously in Se. SP. In addition, it was found that Se was abundantly distributed in the range of pH 4-7 and M-W of 14.4-43 kDa in the 2DE gel. Individuals of Se containing protein should be explored in Se. SP in further studies. 
Selenium enriched Spirulina platensis; Spectral Analysis; Two dimensional electrophoresis; Selenium containing proteins; Proteomics 
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY