Jump to main content
US EPA
United States Environmental Protection Agency
Search
Search
Main menu
Environmental Topics
Laws & Regulations
About EPA
Health & Environmental Research Online (HERO)
Contact Us
Print
Feedback
Export to File
Search:
This record has one attached file:
Add More Files
Attach File(s):
Display Name for File*:
Save
Citation
Tags
HERO ID
6724789
Reference Type
Journal Article
Title
Rat liver antioxidant response to iron and copper overloads
Author(s)
Musacco-Sebio, R; Saporito-Magrina, C; Semprine, J; Torti, H; Ferrarotti, N; Castro-Parodi, M; Damiano, A; Boveris, A; Repetto, MG; ,
Year
2014
Is Peer Reviewed?
Yes
Journal
Journal of Inorganic Biochemistry
ISSN:
0162-0134
EISSN:
1873-3344
Publisher
ELSEVIER SCIENCE INC
Location
NEW YORK
Page Numbers
94-100
PMID
24838005
DOI
10.1016/j.jinorgbio.2014.04.014
Web of Science Id
WOS:000337988600011
Abstract
The rat liver antioxidant response to Fe and Cu overloads (0-60 mg/kg) was studied. Dose- and time-responses were determined and summarized by t(1/2) and C-50, the time and the liver metal content for half maximal oxidative responses. Liver GSH (reduced glutathione) and GSSG (glutathione disulfide) were determined. The GSH content and the GSH/GSSG ratio markedly decreased after Fe (58-66%) and Cu (79-81%) loads, with t(1/2) of 4.0 and 2.0 h. The C-50 were in a similar range for all the indicators (110-124 mu g Fe/g and 40-50 mu g Cu/g) and suggest a unique free-radical mediated process. Hydrophilic antioxidants markedly decreased after Fe and Cu (60-75%; t(1/2): 4.5 and 4.0 h). Lipophilic antioxidants were also decreased (30-92%; t(1/2): 7.0 and 5.5 h) after Fe and Cu. Superoxide dismutase (SOD) activities (Cu,Zn-SOD and Mn-SOD) and protein expression were adaptively increased after metal overloads (Cu,Zn-SOD: t(1/2): 8-8.5 h and Mn-SOD: t(1/2): 8.5-8.0 h). Catalase activity was increased after Fe (65%; t(1/2): 8.5 h) and decreased after Cu (26%; t(1/2): 8.0 h), whereas catalase expression was increased after Fe and decreased after Cu overloads. Glutathione peroxidase activity decreased after metal loads by 22-39% with a t(1/2) of 4.5 h and with unchanged protein expression. GSH is the main and fastest responder antioxidant in Fe and Cu overloads. The results indicate that thiol ( -SH) content and antioxidant enzyme activities are central to the antioxidant defense in the oxidative stress and damage after Fe and Cu overloads. (C) 2014 Elsevier Inc. All rights reserved.
Home
Learn about HERO
Using HERO
Search HERO
Projects in HERO
Risk Assessment
Transparency & Integrity