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675520 
Journal Article 
Effects of butyl benzyl phthalate on enzymes related to energy metabolism in testis of mice 
Wu, D; Li, Y; Tang, B; Fang, F; Ding S-m; Yang, X; Yuan J-l 
2007 
0 
Shengtai Duli Xuebao / Asian Journal of Ecotoxicology
ISSN: 1673-5897 
2 
4 
440-444 
To explore the damage mechanism of Bu benzyl phthalate (BBP) to mouse germ cells from the perspective of energy metab., the enzymes related to energy metab. in the testis of mice were tested in this expt. Kunming male mice were used as exptl. animals and five concn. groups were set (mass fraction: 0, 125, 250, 500, 1000 mg·kg-1). Activities of succinate dehydrogenase (SDH), lactate dehydrogenase (LDH) and Ca-Mg-ATPase in testis homogenate and the organ coeff. of mouse testis were detd. with spectrophotometry after gastric perfusion for 30 days. The results showed that compared with the control group, the activities of lactate dehydrogenase, succinate dehydrogenase and Ca-Mg-ATPase in the high-concn. groups (500, 1000 mg·kg-1) decreased significantly (p<0.01). The activities of the three enzymes in the low-concn. group (125 mg·kg-1) did not change significantly (p>0.05). The activities of lactate dehydrogenase in the mid-concn. group (250 mg·kg-1) did not change significantly (p>0.05), while, the activities of succinate dehydrogenase and Ca-Mg ATPase decreased significantly (p<0.05, p<0.01). Compared with the control group, the organ coeffs. of mouse testis in each exposure group decreased slightly, but the difference did not reach significance (p>0.05). The above results showed that high-concn. Bu benzyl phthalate could not only interfere with aerobic metab. and anaerobic metab. of testicular tissue, but also interfere with the energy use of mouse germ cells, which suggested that the obstacles of energy metab. might be the reasons of male reproductive cell damage induced by Bu benzyl phthalate.