Health & Environmental Research Online (HERO)


Print Feedback Export to File
6763534 
Journal Article 
The use of SIBS triblock copolymers in cardiac surgery: in vitro and in vivo studies in comparison with ePTFE 
Rezvova, MA; Ovcharenko, EA; Nikishev, PA; Kostyuk, SV; Antonova, LV; Akent'Eva, TN; Glushkova, TV; Velikanova, YG; Shishkova, DK; Krivkina, EO; Klyshnikov, KY; Kudryavtseva, YA; Barbarash, LS 
2019 
21 
4 
67-80 
Russian 
RSCI:41709387 
Prosthetics of heart valves by polymer has the potential to solve the problems of existing valve device - mechanical and biological. Aim: to investigate of styrene-isobutylene triblock copolymer (SIBS) biocompatibility synthesized by the controlled cation polymerization method in comparison with the polytetrafluoroethylene (ePTFE) used in clinical practice. Materials and methods. SIBS-based films were prepared by casting from a polymer solution; a biocompatibility assessment in vitro was performed using cell cultures, determining cell viability, cell adhesion, and proliferation; the resistance of materials to calcification was determined by the method of calcification in vitro; an in vivo biocompatibility assessment was performed by subcutaneous implantation of the samples to rats; hemocompatibility was determined ex vivo by evaluation of the degree of hemolysis, aggregation and platelet adhesion. Results. The molecular weight of the synthesized polymer was 33,000 g/mol with a polydispersity index of 1.3. In the study of cell adhesion, there was no significant difference (p = 0.20) in the properties of the SIBS polymer (588 cells/mm2) from the properties of the culture plastics (732 cells/mm2), the cell adhesion for the ePTFE material was 212 cells/mm2. The percentage of dead cells on SIBS, ePTFE samples, respectively, was 4.40% and 4.72% (p = 0.93), for culture plastic - 1.16% (p < 0.05). The cell proliferation on the ePTFE surface (0.10%) was significantly lower (p < 0.05) than for SIBS and culture plastics (62.04% and 44.00%). Implantation results (60 days) showed the formation of fibrous capsules with average thicknesses: 42 μm (ePTFE), 58 μm (SIBS). The calcium content of the explanted samples was 0.39 mg/g (SIBS), 1.25 mg/g (ePTFE) and 93.79 mg/g (GA-xenopericardium) (p < 0.05). The hemolysis level of blood red blood cells after contact with SIBS was 0.35%, ePTFE 0.40%, which is below the positive control (p < 0.05). The maximum platelet aggregation by intact platelet-enriched plasma was 8.60%, which was in contact with the SIBS polymer - 18.11%, and ePTFE - 22.74%. Conclusion. The polymer SIBS when comparing the biocompatibility properties is not inferior to ePTFE and has the potential for use in the development of polymeric prosthetic heart valves new generation. © 2019 Russian Transplant Society. All rights reserved.