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HERO ID
6772188
Reference Type
Journal Article
Title
Lead reduces depolarization-induced calcium entry in cultured DRG neurons without crossing the cell membrane: Fura-2 measurements
Author(s)
Domann, R; Wunder, L; Busselberg, D
Year
1997
Is Peer Reviewed?
Yes
Journal
Cellular and Molecular Neurobiology
ISSN:
0272-4340
EISSN:
1573-6830
Volume
17
Issue
3
Page Numbers
305-314
Language
English
PMID
9187487
DOI
10.1023/A:1026342318006
Web of Science Id
WOS:A1997XC67600003
Abstract
1. Cultured dorsal root ganglion of rat pups were depolarized by exposure to 50 mM K+ and the rise of [Ca2+]i was measured using fura-2 as an indicator. 2. Lead in the extracellular solution reduced the rise of [Ca2+]i in a concentration-dependent manner, with a threshold concentration of 0.25 microM. More than 80% of the calcium entry was prevented by approximately 5 microM lead. The IC50 and the Hill coefficient were 3.1 microM and 1, respectively. 3. This effect was considered to be due to a reduction of VACCCs, since applications of NMDA did not result in any rise of [Ca2+]i. 4. Since Pb2+ itself changes the fura-2 signal in a typical and characteristic manner, fura-2 is also an indicator for Pb2+. No changes in fura-2 signals were detected when lead (5 microM) was applied for several minutes in the absence of calcium, indicating that Pb2+ did not enter the cells. 5. Thus it is concluded that lead prevents calcium entry by reducing VACCCs but does not cross the cell membrane itself.
Keywords
fura-2; Pb2+ neurotoxicity; dorsal root ganglia; cell cultures
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