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HERO ID
6795086
Reference Type
Journal Article
Title
Simultaneous Determination of Atenolol and Chlorthalidone by LC-MS-MS in Human Plasma
Author(s)
Khuroo, A; Mishra, S; Singh, O; Saxena, S; Monif, T; ,
Year
2008
Is Peer Reviewed?
Yes
Journal
Chromatographia
ISSN:
0009-5893
EISSN:
1612-1112
Publisher
VIEWEG
Location
WIESBADEN
Page Numbers
721-729
DOI
10.1365/s10337-008-0755-7
Web of Science Id
WOS:000260524400006
Abstract
A simple, sensitive, selective and rapid liquid chromatography-tandem mass spectrometry method was developed and validated for the simultaneous separation and quantitation of atenolol and chlorthalidone in human plasma using metoprolol and hydrochlorothiazide as internal standard. Following solid phase extraction, the analytes were separated by an isocratic mobile phase on a reversed-phase C-18 column and analyzed by MS in the multiple reaction-monitoring mode (atenolol in positive and chlorthalidone in the negative ion mode). The limit of quantitation for this method was 10 and 15 ng mL(-1) and the linear dynamic range was generally 10-2,050 ng mL(-1) and 15-3,035 ng mL(-1) for atenolol and chlorthalidone, respectively.
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