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6795086 
Journal Article 
Simultaneous Determination of Atenolol and Chlorthalidone by LC-MS-MS in Human Plasma 
Khuroo, A; Mishra, S; Singh, O; Saxena, S; Monif, T; , 
2008 
Yes 
Chromatographia
ISSN: 0009-5893
EISSN: 1612-1112 
VIEWEG 
WIESBADEN 
721-729 
WOS:000260524400006 
A simple, sensitive, selective and rapid liquid chromatography-tandem mass spectrometry method was developed and validated for the simultaneous separation and quantitation of atenolol and chlorthalidone in human plasma using metoprolol and hydrochlorothiazide as internal standard. Following solid phase extraction, the analytes were separated by an isocratic mobile phase on a reversed-phase C-18 column and analyzed by MS in the multiple reaction-monitoring mode (atenolol in positive and chlorthalidone in the negative ion mode). The limit of quantitation for this method was 10 and 15 ng mL(-1) and the linear dynamic range was generally 10-2,050 ng mL(-1) and 15-3,035 ng mL(-1) for atenolol and chlorthalidone, respectively.