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6824677 
Journal Article 
Degradation of pyrene and characterization of Saccharothrix sp PYX-6 from the oligotrophic Tianchi Lake in Xinjiang Uygur Autonomous Region, China 
Hu, YT; Ren, FH; Zhou, PJ; Xia, M; Liu, SJ 
2003 
Yes 
Chinese Science Bulletin
ISSN: 1001-6538
EISSN: 1861-9541 
SCIENCE CHINA PRESS 
BEIJING 
48 
20 
2210-2215 
English 
WOS:000186305600013 
Bacterial strain PYX-6 that utilizes anthracene, phenanthrene, or pyrene for carbon and energy sources for growth was isolated from a non-polluted lake (Tianchi Lake) in Xinjiang Uygur Autonomous Region of China. Its morphology, physiological and biochemical properties, cell wall pattern and G+C mol% content of DNA molecules were characterized. The 16S rRNA gene of strain PYX-6 was sequenced and analyzed for similarities to related bacterial species. Results indicated that strain PYX-6 is a member of the Genus Saccharothrix, and the strain was named Saccharothrix sp. PYX-6. When pyrene was the sole carbon source in cultural medium, the strain PYX-6 assimilated pyrene for growth and 0.005% of yeast extract stimulated pyrene degradation and assimilation. The optimal pH of cultural medium and the optimal shaking frequency during cultivation were 6-8 and 200 r/m, respectively. It was found that the disappearance of pyrene in medium occurred before significant growth of strain PYX-6 took place. Phthalic acid, benzylacetic acid, and benzylpropenoic acid were detected as catabolic intermediates during pyrene degradation with mass spectroscopy and this result indicated that Saccharothrix sp. PYX-6 adopted a pathway that is different from the pathway of the previously reported pyrene-degrading Mycobacterium sp. PYR-1. 
Saccharothrix; biodegradation; polycyclic aromatic hydrocarbon; phthalic acid