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HERO ID
6826765
Reference Type
Journal Article
Title
Fatty acids-based microemulsion liquid chromatographic determination of multiple caffeoylquinic acid isomers and caffeic acid in honeysuckle sample
Author(s)
Ye, LH; Du, LJ; Cao, J
Year
2019
Is Peer Reviewed?
Yes
Journal
Journal of Pharmaceutical and Biomedical Analysis
ISSN:
0731-7085
EISSN:
1873-264X
Volume
171
Page Numbers
22-29
Language
English
PMID
30959316
DOI
10.1016/j.jpba.2019.03.064
Web of Science Id
WOS:000468555900004
Abstract
A green and efficient microemulsion liquid chromatographic (MELC) method using fatty acid as co-surfactant and electrochemical detection was established and validated for the determination of four caffeoylquinic acid isomers and caffeic acid in honeysuckle samples. The influences of each individual component within the isocratic oil-in-water (O/W) microemulsion mobile phase were systematically investigated, such as the type and concentration of co-surfactant, concentration of sodium dodecyl sulphate (SDS), organic modifier addition, type and concentration of oil phase, pH and detection voltage. Results indicated that excellent resolution was achieved using 3.0% w/v of propionic acid, 0.5% w/v of ethyl acetate, 1.0% w/v of SDS, 5% w/v acetonitrile, 90.5% v/v of water and 25 mM sodium dihydrogen phosphate at pH = 3 as microemulsion mobile phase and 0.8 V as the optimal voltage value. Under the optimal condition, analytical performance of developed method was evaluated. The detection limits were below 17.3 ng/mL and intra-day and inter-day precisions by relative standard deviations (RSD%) were between 0.5% and 3.6%. Satisfactory recovery (in the range of 83.8-109.1%) with good repeatability lower than 4.7% (n = 3) was obtained. Therefore, the developed O/W MELC method was rapid, precise and accurate for simultaneous determination of neochlorogenic acid, chlorogenic acid, isochlorogenic acid A and isochlorogenic acid C in honeysuckle samples, with contents of 2.6, 28.7, 18.1 and 5.2 mg/g, respectively.
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