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6837832 
Journal Article 
Three-Way Curve Resolution Applied to the Study of Solvent Effect on the Thermodynamic and Conformational Transitions Related to the Protonation of Polycytidylic Acid 
de Juan, A; Izquierdo-Ridorsa, A; Gargallo, R; Tauler, R; Fonrodona, G; Casassas, E; , 
1997 
Yes 
Analytical Biochemistry
ISSN: 0003-2697
EISSN: 1096-0309 
249 
2 
174-183 
English 
9212869 
WOS:A1997XH22300007 
Solvent effect on the acid-base behavior of polycytidylic acid [poly(C)] is studied by means of potentiometric and spectrometric (UV and CD) procedures. Low-polarity biological environments are mimicked by using a 30% (v/v) dioxane-water mixture. Experiments performed in this medium are compared with previously reported results in aqueous solution in order to determine changes in both thermodynamic and structural aspects associated with modifications of the biomolecular surroundings. Potentiometric and spectrometric studies reveal the presence of a nonlinear polyelectrolytic effect associated with the protonation of poly(C) in the hydroorganic mixture, different from the analogous effect in aqueous solution. The curve resolution method alternating least squares is applied to the poly(C) spectrometric data. Concentration profiles and spectra of both deprotonated poly(C) and half-protonated [poly(C).poly(CH+)] are thus obtained. The fully protonated species poly(CH+) precipitates in the hydroorganic medium at pH values lower than 4. Evidence for the ordered structure of both poly(C) and [poly(C).poly(CH+)] species is seen through the comparison of the macromolecule spectra with those of the cytidine-3',5'-cyclic monophosphate monomer. Polarity decreases around the macromolecule produce significant hypochromicity in the CD spectra and hyperchromicity in the UV spectra, both signs of a disordering effect in the macromolecular structure due to the weakening of base stacking interactions. (C) 1997 Academic Press.<