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6917962 
Journal Article 
Photopolymerized organo-silica hybrid monolithic columns: Characterization of their performance in capillary liquid chromatography 
Weed, AMK; Dvornik, J; Stefancin, JJ; Gyapong, AA; Svec, F; Zajickova, Z; , 
2013 
Yes 
Journal of Separation Science
ISSN: 1615-9306
EISSN: 1615-9314 
WILEY-V C H VERLAG GMBH 
WEINHEIM 
36 
2 
270-278 
English 
23212714 
WOS:000319859500007 
Porous hybrid organo-silica monoliths have been prepared inside pretreated 100 mu m id UV transparent fused-silica capillaries using simultaneous sol-gel transition and polymerization of 3-(methacryloyloxy) propyl trimethoxysilane in the presence of toluene as a porogen. The sol-gel reaction was catalyzed by hydrochloric acid while various photoinitiators including azobisisobutyronitrile, 2,2-dimethoxy-2-phenylacetophenone, and Irgacure 819 were used for the photopolymerization carried out under irradiation with UV light at a wavelength of 254 or 365 nm. The chromatographic performance of photopolymerized monolithic columns in RP liquid chromatographic mode was assessed with respect to the following metrics: column efficiency, methylene and steric selectivity, effect of silanol groups, van Deemter plot, permeability, and pore size distribution. Columns with an efficiency of up to 77 000 plates/m for benzene has been achieved at a flow velocity of 0.47 mm/s. The performance of photopolymerized hybrid monolithic column was compared to the performance of columns prepared via thermally initiated polymerization.