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6935821 
Journal Article 
COMPARISON OF THE PHASE RATIO FOR C18 HPLC COLUMNS USING THREE DIFFERENT ORGANIC MODIFIERS (METHANOL, ETHANOL AND ACETONITRILE) IN MOBILE PHASE COMPOSITION 
Caiali, E; Moldoveanu, SC; David, V; , 
2017 
1 
Revue Roumaine de Chimie
ISSN: 0035-3930 
EDITURA ACAD ROMANE 
BUCURESTI 
629-636 
WOS:000428236200006 
A retention study for five aromatic hydrocarbons was performed on three C18 HPLC columns (two were packed columns and one was based on a monolithic stationary phase) using aqueous mobile phases containing one organic modifier (methanol, ethanol, or acetonitrile) at constant temperature (25 degrees C). Linear dependences between the logarithm of the retention factor and organic modifier percentage in mobile phase were obtained and used to calculate the extrapolated value of the retention factor for mobile phase composed of only water. The value of phase ratio was calculated for each mobile phase composition using a theoretical approach previously developed and based on solvophobic theory (J.Chromatogr. A, 2015, 1381, 194-201). According to this model, the phase ratio depends not only on the nature of the stationary phase, but also on the nature and content of the organic modifier found in the composition of mobile phase. This study showed that the average values of phase ratio calculated over the four pairs of consecutive aromatic hydrocarbons from series benzene/butylbenzene waried in the order of organic modifier, methanol < ethanol < acetonitrile.