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6936665 
Journal Article 
Immobilization of glucose oxidase enzyme (GOD) in large pore ordered mesoporous cage-like FDU-1 silica 
Cides da Silva, LC; Infante, CMC; Lima, AWO; Cosentino, IC; Fantini, MCA; Rocha, FRP; Masini, JC; Matos, , JR; , 
2011 
Yes 
Journal of Molecular Catalysis B: Enzymatic
ISSN: 1381-1177 
ELSEVIER 
AMSTERDAM 
70 
3-4 
149-153 
English 
WOS:000290776600011 
Large pore ordered mesoporous silica FDU-1 with three-dimensional (3D) face-centered cubic, Fm3m arrangement of rnesopores, was synthesized under strong acid media using B-50-6600 poly(ethylene oxide)-poly(butylene oxide)-poly(ethylene oxide) triblock copolymer (EO39BO47EO39), tetraethyl orthosilicate (TEOS) and trimethyl-benzene (TMB). Large pore FDU-1 silica was obtained by using the following gel composition 1TEOS:0.00735B50-6600:0.00735TMB:6HCl:155H(2)O. The pristine material exhibited a BET specific surface area of 684 m(2) g(-1), total pore volume of 0.89 cm(3) g(-1), external surface area of 49 m(2) g(-1) and microporous volume of 0.09 cm(3) g(-1). The enzyme activity was determined by the Flow Injection Analysis-Chemiluminescence (FIA-CL) method. For GOD immobilized on the FDU-1 silica, GOD supernatant and GOD solution, the FIA-CL results were 9.0, 18.6 and 34.0 U, respectively. The value obtained for the activity of the GOD solution with FIA-CL method is in agreement with the 35 U, obtained by spectrophotometry. (C) 2011 Elsevier B.V. All rights reserved. 
FDU-1 silica; Immobilized enzyme activity; Glucose oxidase; Flow injection; Chemiluminescence