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6957711 
Journal Article 
Simultaneous Determination of Resveratrol and Polydatin in Polygonum Cuspidatum by Quantitative Nuclear Magnetic Resonance Spectroscopy 
Yu Shan; Guo Qiang-Sheng; Wang Hui-Lin; Gao Jian-Ping; Xu Xu; , 
2015 
Yes 
Chinese Journal of Analytical Chemistry / Fen Xi Hua Xue
ISSN: 0253-3820 
ELSEVIER SCIENCE INC 
NEW YORK 
69-74 
WOS:000351403000006 
A quantitative nuclear magnetic resonance spectroscopic ( qNMR) method was established for the simultaneous determination of resveratrol and polydatin in Polygonum Cuspidatum traditional Chinese herb cuts and granule. The 2-step ultrasonic extraction method using 80% alcohol and acetone was used for fully extracting these two components in samples before qNMR determination. The qNMR experimental conditions were investigated and deuterated dimethyl sulphoxide-deuterium oxide (10:1, V/ V) was selected as solvent, the pulse delay time was 5 s, the scan number was 32, 2,3,5-triiodobenzoate was used as internal standard which was calibrated with primary standard substance of potassium hydrogen phthalate. The 1H-NMR peaks at 5 6.388-6. 391 ( d, 2H) of resveratrol and 5 6. 322 6. 330 ( t, 1H) of polydatin were chosen as the quantitative peaks. Method validation was performed in terms of precision ( RSD < 0. 6%), linearity ( correlative constants R-2>0. 999), limit of detection (0. 23 g/L for resveratrol and 0. 24 g/L for polydatin) and limit of quantitation ( resveratrol 0. 69 g/L, polydatin 1. 57 g/L), recovery ( resveratrol 97. 7% 103.5%, RSD = 2. 4%, polydatin 94. 5% 99. 2%, RSD = 1. 6%, including the sample extraction and preparaton process). The results showed the reliability of qNMR for traditional Chinese medicine assay. The resveratrol and polydatin in Polygonum Cuspidatum real cuts and granule samples were experimental determined as 3. 57-5. 69 mg/g and 12. 73-24. 07 mg/g, respectively.