US EPA

6969244 

Journal Article 

Assessment of Microbial Diversity of Deyeuxia angustifolia Wetland through Phospholipid Fatty Acids (PLFA) in Sanjiang Plain 

Wang, J; Li, M; Zhang, T; Sui, Xin; Ma, W; Ni, HWei; , 

2018 

Yes 

International Journal of Agriculture and Biology
EISSN: 1560-8530 

FRIENDS SCIENCE PUBL 

FAISALABAD 

1463-1470 

10.17957/IJAB/15.0700 

WOS:000433197100030 

The Sanjiang plain is the most important wetland ecosystem in northeast China. Due to human activity, the water level is severely lowered, resulting in various stages of wetland degradation, with subsequent changes of ecosystem functions. Here we studied the changes in microbial content and diversity of soil in marsh, marsh meadow and meadow wetland, each of these is typically covered by Deyeuxia angustifolia. The method applied was qualitative and quantitative analysis of phospholipid fatty acids (PFLA). 72 types of PLFA were detected in the soil types, of which 29 could be attributed to microbial groups, with prokaryotes being most often detected. The detected characterized PFLA represented a soil microbial biomass of approximately 2 to 31 nmol.g(-1) dry soil. Compared to soil from marsh wetland, the total content of PLFA, and PLFA derived from Gram-positive bacteria and Gram-negative bacteria was higher in meadow and marsh meadow soil. The fatty acid content in bacteria, fungi and protozoavaried between the soil types with lower values in marsh wetlandthan the other soil types. Canonical correlation analysis identified thatsoil pH, water content and total nitrogen content were the most influential factors determining the amount of bacteria in soil, while the nitrate nitrogen content also had an impact. The microbial community structure became more diverseas wetland degradation progressed. (C) 2018 Friends Science Publishers