US EPA

6976804 

Journal Article 

Growth Enhancement and Root Colonization of Rice Seedlings by Rhizobium and Corynebacterium spp 

Naher, UA; Othman, R; Shamsuddin, ZHJ; Saud, HM; Ismail, MR; , 

2009 

Yes 

International Journal of Agriculture and Biology
EISSN: 1560-8530 

FRIENDS SCIENCE PUBL 

FAISALABAD 

586-590 

WOS:000270750200015 

Diazotrophs Form natural association with rice plant. An in vitro experiment was conducted to study the colonization and association of Sb16 and Sb26 diazotrophic strain (previously isolated from rice rhizosphere) on two rice genotypes namely Mayang Segumpal and MR219. Root colonization was observed under scanning and transmission electron microscope. After 5 days of inoculation diazotrophs colonized on the surfaces and internals of lateral roots, root hairs and epidermal cells of the rice roots. Sb16 (Rhizobium sp.) formed aggregated cells and produced mucilaginous materials that may be involved in their attachment oil roots. Rice seedlings inoculated with diazotrophs produced significantly higher biomass compared to control and 35 kg ha(-1) of nitrogen treatments. The diazotrophs association increased rice seedlings root and shoot biomass. Mayang Segumpal rice colonized by Rhizobium sp. (SB16) content higher tissue nitrogen (4.47%) and increased plant biomass by 36% over the non-inoculated control and 22% over 35 kg ha(-1) of N fertilizer. While the MR219 rice inoculated with Corynebacterium sp. contained 4.30% tissue N and increased 32% of plant biomass over non-inoculated control and 21% over 35 kg N ha(-1) The study showed that inoculation with diazotrophic strains (Sb16 & Sb26) improve plant growth including tissue nitrogen content of rice and differences in the association between diazotrophs and rice genotypes.