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HERO ID
6985093
Reference Type
Journal Article
Title
Isolation and identification of infectious bronchitis virus from chickens in Sichuan, China
Author(s)
Wang, HN; Wu, QZ; Huang, Y; Liu, P
Year
1997
Is Peer Reviewed?
Yes
Journal
Avian Diseases
ISSN:
0005-2086
EISSN:
1938-4351
Volume
41
Issue
2
Page Numbers
279-282
DOI
10.2307/1592178
Web of Science Id
WOS:A1997XF45200003
URL
https://www.jstor.org/stable/1592178?origin=crossref
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Abstract
A nonhemagglutinating virus was isolated from kidneys and lungs of chickens suspected of having infectious bronchitis infection. Specific-pathogen-free embryonated chicken eggs were used as the cultural system. With the use of the ciliary activity of chicken embryo tracheal organ cultures as indicator system, the physicochemical properties of one of the isolated strains (SAIB(3)) were shown to be similar to infectious bronchitis virus (IBV) strain M-41 (standard strain); whereas electron microscopy of the isolate showed coronavirus particles. Virus-neutralization tests were performed in trachea organ cultures to compare the serotypes of five IBV isolates and six known IBV strains on the basis of reciprocal neutralization titers and euclidean distance. The cross-neutralization pattern indicated that one isolate was of the T-strain IBV serotype, another of the M-41 IBV serotype, while others had partial serotype relationship to M-41 and T-strains of IBV.
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