Health & Environmental Research Online (HERO)


Print Feedback Export to File
6985093 
Journal Article 
Isolation and identification of infectious bronchitis virus from chickens in Sichuan, China 
Wang, HN; Wu, QZ; Huang, Y; Liu, P 
1997 
Yes 
Avian Diseases
ISSN: 0005-2086
EISSN: 1938-4351 
41 
2 
279-282 
WOS:A1997XF45200003 
A nonhemagglutinating virus was isolated from kidneys and lungs of chickens suspected of having infectious bronchitis infection. Specific-pathogen-free embryonated chicken eggs were used as the cultural system. With the use of the ciliary activity of chicken embryo tracheal organ cultures as indicator system, the physicochemical properties of one of the isolated strains (SAIB(3)) were shown to be similar to infectious bronchitis virus (IBV) strain M-41 (standard strain); whereas electron microscopy of the isolate showed coronavirus particles. Virus-neutralization tests were performed in trachea organ cultures to compare the serotypes of five IBV isolates and six known IBV strains on the basis of reciprocal neutralization titers and euclidean distance. The cross-neutralization pattern indicated that one isolate was of the T-strain IBV serotype, another of the M-41 IBV serotype, while others had partial serotype relationship to M-41 and T-strains of IBV.