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6985721 
Journal Article 
Canine respiratory coronavirus employs caveolin-1-mediated pathway for internalization to HRT-18G cells 
Cerbulo-Vazquez, A; Arriaga-Pizano, L; Cruz-Cureno, G; Bosco-Garate, I; Ferat-Osorio, E; Pastelin-Palacios, R; Figueroa-Damian, R; Castro-Eguiluz, D; Szczepanski, A; Owczarek, K; Milewska, A; Baster, Z; Rajfur, Z; Mitchell, JA; Pyrc, K 
2018 
1 
Veterinary Research
ISSN: 0928-4249 
49 
English 
29970183 
WOS:000437435100002 
Canine respiratory coronavirus (CRCoV), identified in 2003, is a member of the Coronaviridae family. The virus is a betacoronavirus and a close relative of human coronavirus OC43 and bovine coronavirus. Here, we examined entry of CRCoV into human rectal tumor cells (HRT-18G cell line) by analyzing co-localization of single virus particles with cellular markers in the presence or absence of chemical inhibitors of pathways potentially involved in virus entry. We also targeted these pathways using siRNA. The results show that the virus hijacks caveolin-dependent endocytosis to enter cells via endocytic internalization.