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6987236 
Journal Article 
A Vaccine Displaying a Trimeric Influenza-A HA Stem Protein on Capsid-Like Particles Elicits Potent and Long-Lasting Protection in Mice 
Thrane, S; Aves, K; Uddback, I; Janitzek, CM; Han, J; Yang, YR; Ward, AB; Theander, TG; Nielsen, MA; Salanti, Ali; Thomsen, AR; Christensen, J; Sander, AE 
2020 
8 
3 
English 
32679905 
WOS:000580731600001 
Due to constant antigenic drift and shift, current influenza-A vaccines need to be redesigned and administered annually. A universal flu vaccine (UFV) that provides long-lasting protection against both seasonal and emerging pandemic influenza strains is thus urgently needed. The hemagglutinin (HA) stem antigen is a promising target for such a vaccine as it contains neutralizing epitopes, known to induce cross-protective IgG responses against a wide variety of influenza subtypes. In this study, we describe the development of a UFV candidate consisting of a HAstem trimer displayed on the surface of rigid capsid-like particles (CLP). Compared to soluble unconjugated HAstem trimer, the CLP-HAstem particles induced a more potent, long-lasting immune response and were able to protect mice against both homologous and heterologous H1N1 influenza challenge, even after a single dose. 
universal influenza vaccine; HA-stem antigen; capsid-like particles; Virus-like particles; trimer display; IgG2a; pandemic preparedness