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HERO ID
6987487
Reference Type
Journal Article
Title
A strip test for the optical determination of influenza virus H3 subtype using gold nanoparticle coated polystyrene latex microspheres
Author(s)
Liu, X; Yang, J; Li, Q; Wang, Y; Wang, Y; Li, Ge; Shi, J; Ding, P; Guo, J; Deng, R; Zhang, G
Year
2020
Is Peer Reviewed?
1
Journal
Microchimica Acta
ISSN:
0026-3672
EISSN:
1436-5073
Volume
187
Issue
5
Language
English
PMID
32356232
DOI
10.1007/s00604-020-04255-1
Web of Science Id
WOS:000531316300002
Abstract
A strip test is described for the optical determination of influenza virus H3 subtype. It utilizes gold nanoparticle (AuNP) coated polystyrene latex microspheres (PS) as the label and a sandwich format. The AuNP and PS particles were linked using monoclonal antibodies against influenza virus as the bridge. Under the optimal conditions, the visual detection limit of the AuNP-PS-based strip test was as low as 1/16 hemagglutination unit (HAU). It was 64 times higher than that of 10 nm (4 HAU) AuNP-based strip tests. Quantitative analysis showed that the detection limit of the AuNP-PS-based strip is 0.016 HAU. The AuNP-PS-based strip test showed no cross-reactivity to the other subtypes (H1, H5, H7, or H9) of influenza viruses. Graphical abstract .
Keywords
Lateral flow immunoassay; Nanocomposite; Influenza virus; Point-of-care test; Visual determination
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