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6987799 
Journal Article 
Melatonin mediates monochromatic light-induced proliferation of T/B lymphocytes in the spleen via the membrane receptor or nuclear receptor 
Xiong, J; Wang, Z; Cao, J; Dong, Y; Chen, Y 
2020 
1 
Poultry Science
ISSN: 0032-5791
EISSN: 1525-3171 
99 
9 
4294-4302 
English 
32867973 
WOS:000568412000021 
Our studies found that melatonin mediates the monochromatic light-induced lymphocyte proliferation in chickens. However, melatonin receptor subtypes contain membrane receptor (Mel1a/Mel1b/Mel1c) and nuclear receptor (Retinoic acid receptor-related orphan receptor [ROR] alpha/ROR beta/ROR gamma) and are characteristic with cell specificity. This study compared receptor pathway of melatonin, which mediated the monochromatic light-induced T/B lymphocyte proliferations in chickens. Newly hatched chicks were randomly divided into white light, red light, green light (GL), and blue light groups. Green light promoted the membrane receptor expression in the spleen but decreased the nuclear receptor level compared with that of red light. These changes were accompanied by increase of T/B lymphocyte proliferation and plasma melatonin level under GL. Pinealectomy reversed aforementioned changes and resulted in no differences among the light-treated groups. Supplementation of exogenous melatonin enhanced GL-induced T/B lymphocyte proliferation in the spleen but was reversed by Mel1c antagonist prazosin and ROR alpha agonist SR1078 and enhanced by ROR alpha antagonist SR3335. However, Mel1b antagonist 4P-PDOT and ROR gamma antagonist GSK increased the stimulation effect of melatonin on GL-induced T lymphocyte proliferation but no effect on the B-lymphocyte proliferation. These results indicate that melatonin promotes the GL-induced T lymphocyte proliferation through Mel1b, Mel1c, and ROR alpha/ROR gamma; however, the Mel1a, Mel1c, and ROR alpha may be involved in the B lymphocyte proliferation. 
T-lymphocyte; B-lymphocyte; melatonin receptor; lymphocyte proliferation; monochromatic light