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6990998 
Meetings & Symposia 
Abstract 
Bioaccumulation of hydrophobic linear siloxane compounds in rainbow trout (Oncorhynchus mykiss) 
Cantu, N; Gobas, F 
2019 
English 
Given the wide use of hydrophobic siloxane compounds, more care is being taken by legislators around to globe to regulate any potential bioaccumulative and toxic substances in the environment. Likewise, the abundance of the global production of siloxanes demonstrates the importance of these bioaccumulation studies and how best to aid in the addition of the knowledge of how these compounds interact in the environment. Also, with the current guidelines set by government bodies, only one chemical is tested at a time. The present proposal aims to help prove the importance of chemical mixture studies in the hopes of showing the complex nature of a cocktail of chemicals within the environment and any given organism that comes in to contact with said chemicals. The main objective of this study has been to determine in vivo and in vitro biotransformation rates of various siloxanes in rainbow trout fish. Alongside these, concentration-dependent biotransformation will be assessed by reviewing the depuration rate constant over time at varying concentrations to determine if KT is dependent on the concentration of siloxanes within the organism. Preliminary results have shown varying results between the cyclic and linear siloxanes. Specific plans for these studies are as follows: (1) run individual siloxane exposures to juvenile rainbow trout fish (D4, D5, D6, L3, L4, L5) at a concentration of 4.5mM siloxane/g food for and update duration lasting 21 days. Since the depuration rates for the cyclical siloxanes are very slow, and through previous work done in our lab, this high concentration should suffice to represent a depuration rate value, this will also save time and cost for the project. Also, the depuration phase will be extended out further than that of the linear siloxanes. (2) After analysis of linear samples have been completed, subsequent studies will be run at lower and varying concentration to determine if depletion rates are concentration-dependent. (3) The final in vivo experiment will be run as a mixture of all 6 siloxane compounds at various concentrations to determine if the presence of multiple compounds will alter the depletion rate of other compounds. 
November 
SETAC North American 40th Annual Meeting 
Toronto, ON 
November 3-7, 2019