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7003760 
Journal Article 
Optimization and evaluation of an influenza A (H5) pseudotyped lentiviral particle-based serological assay 
Garcia, JM; Lagarde, N; Ma, ESK; de Jong, MD; Peiris, JSM; , 
2010 
Yes 
Journal of Clinical Virology
ISSN: 1386-6532 
ELSEVIER 
AMSTERDAM 
29-33 
19897409 
WOS:000273111200007 
Background: Novel serological methods provide alternative options for sero-diagnosis, sero-epidemiology and for determining evidence of naturally acquired or vaccine induced immunity. Micro-neutralization tests are currently the gold standard for serological studies of highly pathogenic avian influenza in mammalian species but require handling live virus in a biosafety level (BSL) 3 environment. We previously reported the use of H5 pseudotyped lentiviral particles (H5pp) as an alternative to micro-neutralization tests in a BSL-2 setting (Nefkens et al., 2007).Objective: To optimize and evaluate this newly developed H5pp assay on relevant clinical specimens. Study design: We optimise and evaluate the performance of the H5pp assay using well-characterized sera from humans with confirmed H5N1 disease or controls.Results: The H5pp assay is a reliable serological method for the detection and quantification of neutralizing antibody to H5-viruses.Conclusion: H5pp provide a reliable and safe alternative for sero-diagnosis and sero-epidemiology of H5N1 infections in a BSL-2 setting. (C) 2009 Elsevier B.V. All rights reserved.