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HERO ID
7007910
Reference Type
Journal Article
Title
Cell culture-grown putative bovine respiratory torovirus identified as a coronavirus
Author(s)
Cornelissen, L; van Woensel, PAM; de Groot, RJ; Horzinek, MC; Visser, N; Egberink, HF; ,
Year
1998
Is Peer Reviewed?
1
Journal
Veterinary Record
ISSN:
0042-4900
EISSN:
2042-7670
Publisher
BMJ PUBLISHING GROUP
Location
LONDON
Page Numbers
683-686
PMID
9670455
Web of Science Id
WOS:000074538800006
Abstract
A putative bovine respiratory torovirus (BRTV) was propagated in bovine fetal diploid lung and human colonic tumour cells, and fringed pleomorphic particles were detected in the culture supernatants by electron microscopy. Antisera directed against a bovine (Breda strain) and equine (Berne strain) torovirus failed to react with BRTV-infected cells in immunofluorescence assays and did not neutralise BRTV. No toroviral RNA was found in the supernatants of infected cells by means of a reverse transcriptase-polymerase chain reaction with torovirus-specific primers. On the other hand, bovine coronavirus-specific antisera and monoclonal antibodies did neutralise the cytopathic effects, and coronaviral antigen was detected in the cultures by immunofluorescence, Furthermore, bovine coronavirus RNA was detected in the supernatants of BRTV-infected cells after nucleic acid amplification, It is concluded that the cytopathic BRTV isolate is a coronavirus.
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