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Citation
Tags
HERO ID
7020706
Reference Type
Journal Article
Title
Activation of liver X receptor induces macrophage interleukin-5 expression
Author(s)
Chen, Y; Duan, Y; Kang, Y; Yang, X; Jiang, M; Zhang, L; Li, G; Yin, Z; Hu, W; Dong, P; Li, X; Hajjar, DP; Han, J; ,
Year
2012
Is Peer Reviewed?
Yes
Journal
Journal of Biological Chemistry
ISSN:
0021-9258
EISSN:
1083-351X
Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
Location
BETHESDA
Volume
287
Issue
52
Page Numbers
43340-43350
Language
English
PMID
23150660
DOI
10.1074/jbc.M112.403394
Web of Science Id
WOS:000312940800015
Abstract
IL-5 stimulates production of T15/EO6 IgM antibodies that can block the uptake of oxidized low density lipoprotein by macrophages, whereas a deficiency in macrophage IL-5 expression accelerates development of atherosclerosis. Liver X receptors (LXRs) are ligand-activated transcription factors that can induce macrophage ABCA1 expression and cholesterol efflux, thereby inhibiting the development of atherosclerosis. However, it remains unknown whether additional mechanisms, such as the regulation of macrophage IL-5 expression, are related to the anti-atherogenic properties of LXR. We initially defined IL-5 expression in macrophages where the LXR ligand (T0901317) induced macrophage IL-5 protein expression and secretion. The overexpression of LXR increased, whereas its knockdown inhibited IL-5 expression. Furthermore, we found that LXR activation increased IL-5 transcripts, promoter activity, formation of an LXR·LXR-responsive element complex, and IL-5 protein stability. In vivo, we found that T0901317 increased IL-5 and total IgM levels in plasma and IL-5 expression in multiple tissues in wild type mice. In LDL receptor knock-out (LDLR(-/-)) mice, T0901317 increased IL-5 expression in the aortic root area. Taken together, our studies demonstrate that macrophage IL-5 is a target gene for LXR activation, and the induction of macrophage IL-5 expression can be related to LXR-inhibited atherosclerosis.
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