p53 and its 14 kDa C-terminal domain recognize primary DNA damage in the form of insertion/deletion mismatches | Health & Environmental Research Online (HERO)

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Citation
Tags

HERO ID

7049498

Reference Type

Journal Article

Title

p53 and its 14 kDa C-terminal domain recognize primary DNA damage in the form of insertion/deletion mismatches

Author(s)

Lee, S; Elenbaas, B; Levine, A; Griffith, J; ,

Year

1995

Is Peer Reviewed?

Yes

Journal

Cell
ISSN: 0092-8674
EISSN: 1097-4172

Language

English

PMID

7600570

DOI

10.1016/s0092-8674(05)80006-6

Abstract

Insertion/deletion (IDL) mismatches in DNA are lesions consisting of extra bases on one strand. Here, the binding of p53 and its 14 kDa C-terminal domain to DNAs containing one or three 3-cytosine IDL mismatches was examined. Electron microscopy showed that both p53 forms bound predominantly as tetramers at the lesions while single-stranded binding proteins did not bind. Gel retardation assays showed that p53 formed highly stable complexes when the DNA contained the IDL mismatches, but only unstable complexes when the DNA lacked lesions (but did contain free ends). The highly stable complexes had a half-life of > 2 hr, suggesting that upon encountering lesions, p53 may recruit other proteins to the site, providing a signal for DNA damage.