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HERO ID
7050017
Reference Type
Journal Article
Title
The origin and maintenance of mammalian peroxisomes involves a de novo PEX16-dependent pathway from the ER
Author(s)
Kim, PK; Mullen, RT; Schumann, U; Lippincott-Schwartz, J; ,
Year
2006
Is Peer Reviewed?
Yes
Journal
Journal of Cell Biology
ISSN:
0021-9525
EISSN:
1540-8140
Language
English
PMID
16717127
DOI
10.1083/jcb.200601036
Abstract
Peroxisomes are ubiquitous organelles that proliferate under different physiological conditions and can form de novo in cells that lack them. The endoplasmic reticulum (ER) has been shown to be the source of peroxisomes in yeast and plant cells. It remains unclear, however, whether the ER has a similar role in mammalian cells and whether peroxisome division or outgrowth from the ER maintains peroxisomes in growing cells. We use a new in cellula pulse-chase imaging protocol with photoactivatable GFP to investigate the mechanism underlying the biogenesis of mammalian peroxisomes. We provide direct evidence that peroxisomes can arise de novo from the ER in both normal and peroxisome-less mutant cells. We further show that PEX16 regulates this process by being cotranslationally inserted into the ER and serving to recruit other peroxisomal membrane proteins to membranes. Finally, we demonstrate that the increase in peroxisome number in growing wild-type cells results primarily from new peroxisomes derived from the ER rather than by division of preexisting peroxisomes.
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