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7053028 
Journal Article 
Identification of the versatile scaffold protein RACK1 on the eukaryotic ribosome by cryo-EM 
Sengupta, J; Nilsson, J; Gursky, R; Spahn, CM; Nissen, P; Frank, J; , 
2004 
1 
Nature Structural & Molecular Biology
ISSN: 1545-9993
EISSN: 1545-9985 
English 
15334071 
RACK1 serves as a scaffold protein for a wide range of kinases and membrane-bound receptors. It is a WD-repeat family protein and is predicted to have a beta-propeller architecture with seven blades like a Gbeta protein. Mass spectrometry studies have identified its association with the small subunit of eukaryotic ribosomes and, most recently, it has been shown to regulate initiation by recruiting protein kinase C to the 40S subunit. Here we present the results of a cryo-EM study of the 80S ribosome that positively locate RACK1 on the head region of the 40S subunit, in the immediate vicinity of the mRNA exit channel. One face of RACK1 exposes the WD-repeats as a platform for interactions with kinases and receptors. Using this platform, RACK1 can recruit other proteins to the ribosome.