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7058228 
Journal Article 
NEGATIVE EFFECT OF PATULIN ON RABBIT BLOOD AND ITS MODULATION BY EPICATECHIN 
Zbynovska, K; Bovdisova, I; Kalafova, A; Ondruska, L; Jurcik, R; Chrastinova, L; Tusimova, Eva; Kovacik, A; Capcarova, M; , 
2016 
MENDEL UNIV BRNO 
BRNO 
308-316 
WOS:000400583400047 
In this study we aimed to investigate the effect of patulin in low dose separately or in combination with three different concentrations of epicatechin on haematological parameters in rabbit blood in vivo. Animals (adult female rabbits, body weight 4 +/- 0.5 kg; n=20) were divided into five groups: control group (C) and experimental groups which received patulin two times in week intramuscularly separately in concentration 10 mu g/kg (E1) or in combination with epicatechin which was received also intramuscularly three times a week in concentration 10 mu g/kg (E2), 100 mu g/kg (E3) and 1000 mu g/kg (E4) for five weeks. During the experiment four collections of blood was obtained and haematological parameters were analysed (WBC - total white blood cell count, LYM - lymphocyte count, MID - medium size cell count, RBC - red blood cell count, HGB - haemoglobin, HCT - haematocrit, PLT - platelet count) using haematology analyser Abacus junior VET (Diatron MI LtD., Budapest, Hungary). We observed low count of LYM in group where we added only patulin in low dose (E1) after two, four weeks and also at the end of the experiment. In the experimental group, where we added epicatechin in the lowest concentration in our experiment in combination with patulin (E2), the results were similar like in E1 experimental group but at the end of the experiment the values of LYM in E2 experimental group were in normal reference range. In the experimental groups where we added the middle and the highest concentration of epicatechin in our experiment, LYM were not affected from the beginning to the end of the experiment. So we can suppose protective role of epicatechin against negative effect of patulin on LYM in rabbit blood in vivo. The other analysed haematological parameters were not affected by treatment with patulin separately or in combination with epicatechin. 
Pavlik, A; Slama, P; Skarpa, P; 
12th International Scientific Conference on Animal Physiology 
Boretice, CZECH REPUBLIC